Dissecting the molecular basis underlying mycobacterial cell-wall hydrolysis by the catalytic domains of D29LysA and DS6ALysA phage endolysins.

Ceballos-Zúñiga F, Galvez-Larrosa L, Muñoz IG, Infantes L, Fernández-Carrillo J, Pérez-Dorado I
Int J Biol Macromol :148896 (2025 Nov 7)
PMID: 41207583
doi: 10.1016/j.ijbiomac.2025.148896 		Submitted to SASBDB: 2025 Jan 21
Published in SASBDB:

SASDWN5 – N-acetylmuramoyl-L-alanine amidase (without His-Tag)

N-acetylmuramoyl-L-alanine amidase experimental SAS data
COOT model
Sample: N-acetylmuramoyl-L-alanine amidase monomer, 43 kDa Mycobacterium phage DS6A protein
Buffer: 20 mM Tris, 150 mM NaCl and 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 30
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 50 nm3

SASDWP5 – N-acetylmuramoyl-L-alanine amidase (with His-Tag)

N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) experimental SAS data
COOT model
Sample: N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) monomer, 45 kDa protein
Buffer: 20 mM Tris, 150 mM NaCl and 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 30
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 62 nm3