A new protein-dependent riboswitch activates ribosomal frameshifting

Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
(2025 Jul 18)

doi: 10.1101/2025.07.17.665365 		Submitted to SASBDB: 2025 Apr 1
Published in SASBDB:

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer: 20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWR5 – Stimulatory element RNA

Stimulatory element RNA experimental SAS data
SREFLEX model
Sample: Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 19 nm3

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3