A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses

Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
Molecular Cell (2026 Aug)

doi: 10.1016/j.molcel.2026.07.036 		Submitted to SASBDB: 2025 Apr 1
Published in SASBDB:

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer: 20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWR5 – Stimulatory element RNA

Stimulatory element RNA experimental SAS data
SREFLEX model
Sample: Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 19 nm3

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3