Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.

Zhou W, Strmšek Ž, Snoj J, Škarabot M, Jerala R
Small 21(30):e2502060 (2025 Jul)

PMID: 40442952 doi: 10.1002/smll.202502060

Submitted to SASBDB: 2025 Feb 6 Published in SASBDB: 2025 Aug 1

SASDW37 – SSRD - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24 RgGuinier 4.4 nm Dmax 13.6 nm VolumePorod 300 nm3

SASDW47 – SSRD-2xDRSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-RFP-St Extension Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein DCV-RFP-St monomer, 41 kDa synthetic construct protein DCV-RFP-St monomer, 41 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24 RgGuinier 5.1 nm Dmax 15.4 nm VolumePorod 480 nm3

SASDW57 – SSRD-2xDGSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-GFP-St Extension Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein DCV-GFP-St monomer, 41 kDa synthetic construct protein DCV-GFP-St monomer, 41 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18 RgGuinier 5.8 nm Dmax 17.3 nm VolumePorod 580 nm3

SASDW67 – DRSt - Chimeric protein consisting of DCV, RFP and SpyTag domain Sample: DCV-RFP-St monomer, 41 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24 RgGuinier 2.8 nm Dmax 9.2 nm VolumePorod 60 nm3

SASDW77 – DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18 RgGuinier 2.8 nm Dmax 9.1 nm VolumePorod 54 nm3

SASDW87 – DGSt-LucSc - DCV-GFP-SpyTag and NanoLuc-SpyCatcher Conjugate via SpyCatcher-SpyTag Reaction Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein NanoLuc-Sc monomer, 30 kDa synthetic construct protein Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18 RgGuinier 3.4 nm Dmax 12.5 nm VolumePorod 105 nm3