A ternary switch model governing ERα ligand binding domain conformation.

McDougal DP, Pederick JL, Novick SJ, Jovcevski B, Warrender AK, Pascal BD, Griffin PR, Bruning JB
Nat Commun 16(1):10363 (2025 Nov 24)
PMID: 41285747
doi: 10.1038/s41467-025-65323-9 		Submitted to SASBDB: 2025 Feb 6
Published in SASBDB:

SASDWC7 – SEC-SAXS data of wildtype human apo estrogen receptor alpha ligand binding domain (LBD) at pH 7.5

Estrogen receptor alpha experimental SAS data
DAMMIF model
Sample: Estrogen receptor alpha dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5% glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Nov 9
RgGuinier 2.4 nm
Dmax 7.4 nm
VolumePorod 74 nm3

SASDWD7 – SEC-SAXS data of wildtype Melanotaenia fluviatilis apo estrogen receptor alpha ligand binding domain (LBD) at pH 7.5

Estrogen receptor alpha experimental SAS data
DAMMIF model
Sample: Estrogen receptor alpha dimer, 57 kDa Melanotaenia fluviatilis protein
Buffer: 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5% glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Nov 9
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 72 nm3