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Unreleased project

Rational design of biosensors by computational analysis

Jens Reiners Submitted to SASBDB: 2025 Feb 26

SASDXF3 – RTX cytotoxin pro-MbxA without acylation (pro-MbxA)

RTX cytotoxin pro-MbxA without acylation experimental SAS data
ALPHAFOLD model
Sample: RTX cytotoxin pro-MbxA without acylation monomer, 100 kDa Moraxella bovis protein
Buffer: 50 mM Tris, 100 mM NaCl, 10mM CaCl2, pH: 7.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 9
RgGuinier 3.8 nm
Dmax 13.4 nm
VolumePorod 154 nm3

SASDWP9 – Ratiometric matryoshka biosensor for Fe2+ – MDtxRGA_G149

Ratiometric matryoshka biosensor for Fe2+ experimental SAS data
ALPHAFOLD model
Sample: Ratiometric matryoshka biosensor for Fe2+ monomer, 84 kDa synthetic construct protein
Buffer: 20 mM MOPS, 250 mM KCl,, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2024 Nov 15
RgGuinier 3.6 nm
Dmax 12.4 nm
VolumePorod 118 nm3