The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.

Hultman J, Morad V, Tanner E, Kenney TMG, Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
Nat Commun (2026 Feb 24)
PMID: 41735282
doi: 10.1038/s41467-026-69725-1 		Submitted to SASBDB: 2025 Aug 19
Published in SASBDB:

SASDXU7 – N-Myc proto-oncogene protein residues 1-69

N-myc proto-oncogene protein, residues 1-69 experimental SAS data
N-myc proto-oncogene protein, residues 1-69 Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-69 monomer, 8 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 12 nm3

SASDXV7 – N-Myc proto-oncogene protein, residues 1-100

N-myc proto-oncogene protein, residues 1-100 experimental SAS data
N-myc proto-oncogene protein, residues 1-100 Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-100 monomer, 11 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
RgGuinier 3.0 nm
Dmax 13.0 nm
VolumePorod 22 nm3

SASDXW7 – Kinase domain of Aurora kinase A mutant C290A:C393A

Aurora kinase A mutant C290A:C393A experimental SAS data
Aurora kinase A mutant C290A:C393A Kratky plot
Sample: Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
RgGuinier 2.2 nm
Dmax 9.5 nm
VolumePorod 59 nm3

SASDXX7 – N-Myc proto-oncogene residues 1-69 in complex with Aurora kinase A mutant C290A:C393A

N-myc proto-oncogene protein, residues 1-69Aurora kinase A mutant C290A:C393A experimental SAS data
N-myc proto-oncogene protein, residues 1-69 Aurora kinase A mutant C290A:C393A Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-69 monomer, 8 kDa Homo sapiens protein
Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
RgGuinier 2.5 nm
Dmax 10.2 nm
VolumePorod 62 nm3

SASDXY7 – N-Myc proto-oncogene residues 1-100 in complex with Aurora kinase A mutant C290A:C393A

N-myc proto-oncogene protein, residues 1-100Aurora kinase A mutant C290A:C393A experimental SAS data
N-myc proto-oncogene protein, residues 1-100 Aurora kinase A mutant C290A:C393A Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-100 monomer, 11 kDa Homo sapiens protein
Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 9
RgGuinier 2.8 nm
Dmax 11.4 nm
VolumePorod 82 nm3