Structural enzymological studies of the long chain fatty acyl-CoA synthetase FadD5 from the mce1 operon of Mycobacterium tuberculosis.

Rahman MA, Dalwani S, Venkatesan R
Biochem Biophys Res Commun 769:151960 (2025 May 9)

PMID: 40347623 doi: 10.1016/j.bbrc.2025.151960

Submitted to SASBDB: 2025 Mar 6 Published in SASBDB: 2025 Mar 7

SASDX52 – Fatty acyl-CoA synthetase FadD5 Sample: Probable fatty-acid-CoA ligase FadD5 (Fatty-acid-CoA synthetase) (Fatty-acid-CoA synthase) dimer, 123 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM HEPES, 500 mM NaCl, 5 mM MgCl2, 1 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Oct 20 RgGuinier 3.6 nm Dmax 12.3 nm VolumePorod 221 nm3

SASDX62 – Fatty acyl-CoA synthetase FadD5 with 2 mM ATP Sample: Probable fatty-acid-CoA ligase FadD5 (Fatty-acid-CoA synthetase) (Fatty-acid-CoA synthase) dimer, 123 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM HEPES, 500 mM NaCl, 5 mM MgCl2, 1 mM β-mercaptoethanol, 2mM ATP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Oct 20 RgGuinier 3.6 nm Dmax 12.1 nm VolumePorod 213 nm3

SASDX72 – Fatty acyl-CoA synthetase FadD5 with 2 mM coenzyme A (CoA) Sample: Probable fatty-acid-CoA ligase FadD5 (Fatty-acid-CoA synthetase) (Fatty-acid-CoA synthase) dimer, 123 kDa Mycobacterium tuberculosis (strain … protein Buffer: 20 mM HEPES, 500 mM NaCl, 5 mM MgCl2, 1 mM β-mercaptoethanol, 2 mM coenzyme A (CoA), pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Oct 20 RgGuinier 3.7 nm Dmax 12.0 nm VolumePorod 210 nm3