New structural insights into the control of the retinoic acid receptors RAR/RXR by DNA, ligands, and transcriptional coregulators.

Tambones I, Sagar A, Vankova P, Loginov D, Carivenc C, Rochel N, Bourguet W, Man P, Bernadó P, le Maire A
Nucleic Acids Res 53(18) (2025 Sep 23)

PMID: 41036627 doi: 10.1093/nar/gkaf967

Submitted to SASBDB: 2025 Mar 19 Published in SASBDB: 2025 Oct 13

SASDXM2 – RAR/RXR heterodimer : BMS493 : DR0 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein Direct Repeat binding site with zero-base-pair spacing monomer, 10 kDa DNA BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 18 RgGuinier 3.6 nm Dmax 13.1 nm VolumePorod 121 nm3

SASDXN2 – RAR/RXR heterodimer : BMS493 : DR0L element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Direct Repeat binding site with zero-base-pair spacing (long sequence) monomer, 19 kDa DNA Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 12 RgGuinier 4.1 nm Dmax 14.8 nm VolumePorod 152 nm3

SASDXP2 – RAR/RXR heterodimer : BMS493 : DR1 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with one-base-pair spacing monomer, 11 kDa DNA Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 20 RgGuinier 3.8 nm Dmax 13.9 nm VolumePorod 120 nm3

SASDXQ2 – RAR/RXR heterodimer : BMS493 : DR5 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with five-base-pair spacing monomer, 13 kDa DNA Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 12 RgGuinier 3.9 nm Dmax 14.3 nm VolumePorod 130 nm3

SASDXR2 – RAR/RXR heterodimer : BMS493 : IR0 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Inverted Repeat binding site with zero-base-pair spacing monomer, 11 kDa DNA Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 20 RgGuinier 3.9 nm Dmax 12.9 nm VolumePorod 121 nm3

SASDXS2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR0L element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Direct Repeat binding site with zero-base-pair spacing (long sequence) monomer, 19 kDa DNA Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 12 RgGuinier 5.4 nm Dmax 20.1 nm VolumePorod 363 nm3

SASDXT2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR1 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with one-base-pair spacing monomer, 11 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 20 RgGuinier 5.6 nm Dmax 21.8 nm VolumePorod 330 nm3

SASDXU2 – RAR/RXR heterodimer : BMS493 : NCoR NID : DR5 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Direct Repeat binding site with five-base-pair spacing monomer, 13 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 12 RgGuinier 5.7 nm Dmax 21.8 nm VolumePorod 363 nm3

SASDXV2 – RAR/RXR heterodimer : BMS493 : NCoR NID : IR0 element Sample: Retinoic acid receptor alpha monomer, 39 kDa Mus musculus protein BMS493 (RAR inverse agonist ligand) monomer, 0 kDa Retinoid X receptor alpha monomer, 38 kDa Homo sapiens protein Inverted Repeat binding site with zero-base-pair spacing monomer, 11 kDa DNA Nuclear receptor corepressor 1 monomer, 29 kDa Mus musculus protein Buffer: 20 mM Tris HCl, 75 mM NaCl, 75 mM KCl, 2 mM CHAPS, 4 mM MgCl₂, 12 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 20 RgGuinier 5.5 nm Dmax 22.9 nm VolumePorod 257 nm3