Unique structural and ligand-binding properties of the Staphylococcus aureus serine hydrolase FphE

Jo J, Upadhyay T, You X, Bennett J, Lee H, Bogyo M, Fellner M
Proceedings of the National Academy of Sciences 123(13) (2026 Mar 24)

doi: 10.1073/pnas.2532683123 		Submitted to SASBDB: 2025 May 2
Published in SASBDB:

SASDX64 – Carboxylic ester hydrolase FphE

Uncharacterized hydrolase SAUSA300_2518 experimental SAS data
SREFLEX model
Sample: Uncharacterized hydrolase SAUSA300_2518 dimer, 62 kDa Staphylococcus aureus (strain … protein
Buffer: 10 mM HEPES, 50 mM NaCl, 0.1% w/v NaN3, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2025 Mar 26
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 84 nm3

SASDX74 – Carboxylic ester hydrolase FphF

S-formylglutathione hydrolase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: S-formylglutathione hydrolase dimer, 59 kDa Staphylococcus aureus (strain … protein
Buffer: 10 mM HEPES, 50 mM NaCl, 0.1% w/v NaN3, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2025 Mar 26
RgGuinier 2.8 nm
Dmax 9.3 nm
VolumePorod 99 nm3