Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus

Kodesia A, Kapoor S, Thakur K
Journal of Biological Chemistry :110907 (2025 Nov)

doi: 10.1016/j.jbc.2025.110907

Submitted to SASBDB: 2025 May 18 Published in SASBDB: 2025 Nov 7

SASDXP4 – Mutual gliding motility protein C from Myxococcus xanthus Sample: Mutual gliding motility protein C dimer, 32 kDa Myxococcus xanthus (strain … protein Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 2.3 nm Dmax 9.8 nm VolumePorod 49 nm3

SASDXQ4 – C-terminal helix of RomR protein from Myxococcus xanthus Sample: Two-component system response regulator trimer, 26 kDa Myxococcus xanthus protein Buffer: 20 mM HEPES, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 2.7 nm Dmax 10.3 nm VolumePorod 43 nm3

SASDXR4 – C-terminal region of RomR protein from Myxococcus xanthus Sample: Two-component system response regulator trimer, 39 kDa Myxococcus xanthus protein Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 4.1 nm Dmax 16.7 nm VolumePorod 81 nm3

SASDXS4 – Mutual gliding motility protein C interacting with the C-terminal helix of RomR protein from Myxococcus xanthus Sample: GTPase dimer, 32 kDa Myxococcus xanthus (strain … protein Two-component system response regulator trimer, 26 kDa Myxococcus xanthus DK … protein Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 2.8 nm Dmax 11.0 nm VolumePorod 91 nm3

SASDXT4 – Mutual gliding motility protein C interacting with the C-terminal region of RomR protein from Myxococcus xanthus Sample: GTPase dimer, 32 kDa Myxococcus xanthus (strain … protein Two-component system response regulator trimer, 39 kDa Myxococcus xanthus protein Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 2.5 nm Dmax 9.1 nm VolumePorod 79 nm3

SASDXU4 – Mutual gliding motility protein C and B interacting with the C-terminal helix of RomR protein from Myxococcus xanthus Sample: Two-component system response regulator trimer, 26 kDa Myxococcus xanthus DK … protein Mutual gliding motility protein C dimer, 32 kDa Myxococcus xanthus (strain … protein Gliding motility protein MglB dimer, 32 kDa Myxococcus xanthus (strain … protein Buffer: 20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 Mar 31 RgGuinier 3.8 nm Dmax 13.6 nm VolumePorod 204 nm3