Structural, biophysical and biochemical studies of CAZYmes involved in exopolysaccharides degradation of microbial biofilms

Amanda Freitas Cruz
Universidade de São Paulo Master's thesis (2025 Sep 18)

URL: https://doi.org/10.11606/D.76.2025.tde-18092025-082115

Submitted to SASBDB: 2025 May 24 Published in SASBDB: 2025 Nov 24

SASDXX4 – Serratia marcescens poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB Sample: Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB monomer, 73 kDa Serratia marcescens protein Buffer: phosphate-buffered saline, pH: 7.4 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Feb 3 RgGuinier 2.9 nm Dmax 9.4 nm VolumePorod 102 nm3

SASDXY4 – Serratia marcescens truncated Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB Sample: Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB monomer, 69 kDa Serratia marcescens protein Buffer: phosphate-buffered saline, pH: 7.4 Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Feb 3 RgGuinier 2.8 nm Dmax 9.0 nm VolumePorod 102 nm3