Activation of a Secondary-Messenger Receptor via Allosteric Modulation of a Dynamic Conformational Ensemble.

Söldner B, Singh H, Akoury E, Witte G, Linser R
Angew Chem Int Ed Engl :e202509394 (2025 Aug 6)

PMID: 40770896 doi: 10.1002/anie.202509394

Submitted to SASBDB: 2025 Jun 17 Published in SASBDB: 2025 Aug 7

SASDXD5 – S.aureus PstA in a ligand-free state (cyclic-di-AMP receptor) Sample: Cyclic-di-AMP receptor trimer, 42 kDa Staphylococcus aureus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 11 RgGuinier 2.7 nm Dmax 11.0 nm VolumePorod 91 nm3

SASDXE5 – S.aureus PstA with c-di-AMP (cyclic-di-AMP receptor) Sample: Cyclic-di-AMP receptor trimer, 42 kDa Staphylococcus aureus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 11 RgGuinier 2.6 nm Dmax 11.0 nm VolumePorod 86 nm3