A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation - revision

Bao Nguyen Viet

Submitted to SASBDB: 2025 Jul 1 Published in SASBDB: 2025 Sep 3

SASDXJ5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit alpha holoenzyme with Exon 14 as the linker region Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) dodecamer, 620 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 8.1 nm

SASDXK5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 14 as the linker region + 10 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) dodecamer, 620 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.5 nm

SASDXL5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 14 as the linker region + 2000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) dodecamer, 620 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 8.4 nm

SASDXM5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 14 as the linker region + 20000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ329-344) dodecamer, 620 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 9.9 nm

SASDXN5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 6.9 nm

SASDXP5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 10 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.1 nm

SASDXQ5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 100 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.2 nm

SASDXR5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 1000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.6 nm

SASDXS5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 2000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.2 nm

SASDXT5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 5000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 7.8 nm

SASDXU5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 10000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 8.0 nm

SASDXV5 – Calcium/calmodulin-dependent protein kinase type II (CaMKII) subunit holoenzyme with Exon 18 as the linker region + 20000 nM Ca2+/CaM Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (Δ1-6; Q223K; Δ 316-329) dodecamer, 626 kDa Homo sapiens protein Buffer: 50 mM Tris , 0.2 mM CaCl2, 150 mM KCl, 10 mM MgCl2, 2 mM ATP, pH: 8 Experiment: SAXS data collected at Rigaku/BioSAXS-2000 SAXS, Institute for Applied Life Sciences/Biophysical Characterization on 2024 Dec 18 RgGuinier 10.0 nm