Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.

Gandhi S, Vasudevan D
Biochimie (2025 Nov 11)

PMID: 41232797 doi: 10.1016/j.biochi.2025.11.003

Submitted to SASBDB: 2025 Aug 4 Published in SASBDB: 2025 Nov 14

SASDXB7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1) with C terminal His6-tag Sample: Anti-Silencing Function 1 monomer, 28 kDa Entamoeba histolytica strain … protein Buffer: 20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2023 Oct 1 RgGuinier 3.5 nm Dmax 10.1 nm VolumePorod 40 nm3

SASDXC7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex Sample: Anti-Silencing Function 1 monomer, 28 kDa Entamoeba histolytica strain … protein Histone H3 monomer, 15 kDa Entamoeba histolytica strain … protein Histone H4 monomer, 13 kDa Entamoeba histolytica strain … protein Buffer: 20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2023 Oct 1 RgGuinier 2.9 nm Dmax 8.0 nm VolumePorod 84 nm3