Crystal structure of a complete threonyl tRNA synthetase: toggle clamp model?

Tran T, Fenwick M, Edwards T, Dranow D, Abendroth J, Shek R, Tillery L, Craig J, Van Voorhis W, Myler P
Biochemical and Biophysical Research Communications :152677 (2025 Sep)

doi: 10.1016/j.bbrc.2025.152677

Submitted to SASBDB: 2025 Aug 22 Published in SASBDB: 2025 Sep 22

SASDXZ7 – SmThrRS model for open state based on PDB entry 6vu9 Sample: Stenotrophomonas maltophilia threonyl tRNA synthetase dimer, 146 kDa Stenotrophomonas maltophilia (strain … protein Buffer: 20 mM HEPES, 0.2 M NaCl, 1.5% v/v glycerol, and 1 mM TCEP, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Nov 7 RgGuinier 4.2 nm Dmax 16.2 nm

SASDX28 – SmThrRS model for closed state based on PDB entry 1qf6 Sample: Stenotrophomonas maltophilia threonyl tRNA synthetase dimer, 146 kDa Stenotrophomonas maltophilia (strain … protein Buffer: 20 mM HEPES, 0.2 M NaCl, 1.5% v/v glycerol, and 1 mM TCEP, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Nov 7 RgGuinier 4.2 nm Dmax 16.2 nm

SASDX38 – SmThrRS model for closed state based on PDB entry 1nyr Sample: Stenotrophomonas maltophilia threonyl tRNA synthetase dimer, 146 kDa Stenotrophomonas maltophilia (strain … protein Buffer: 20 mM HEPES, 0.2 M NaCl, 1.5% v/v glycerol, and 1 mM TCEP, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Nov 7 RgGuinier 4.2 nm Dmax 16.2 nm

SASDX48 – SmThrRS model based on PDB entries 6vu9 and 1qf6 Sample: Stenotrophomonas maltophilia threonyl tRNA synthetase dimer, 146 kDa protein Buffer: 20 mM HEPES, 0.2 M NaCl, 1.5% v/v glycerol, and 1 mM TCEP, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Nov 7 RgGuinier 4.2 nm