Dynamic assembly of a large multidomain ribozyme visualized by cryo-electron microscopy.

Jadhav S, Maiorca M, Manigrasso J, Saha S, Rakitch A, Muscat S, Mulvaney T, De Vivo M, Topf M, Marcia M
Nat Commun 16(1):10195 (2025 Nov 27)
PMID: 41309593
doi: 10.1038/s41467-025-65502-8 		Submitted to SASBDB: 2025 Sep 15
Published in SASBDB:

SASDX39 – Group IIC Intron Domain 1

Group IIC Intron Domain 1 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1 monomer, 89 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Jul 7
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 188 nm3

SASDX59 – Group IIC intron Domain 1 & 2

Group IIC Intron Domain 1 & 2 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1 & 2 monomer, 96 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
RgGuinier 3.6 nm
Dmax 11.0 nm
VolumePorod 179 nm3

SASDX49 – Group IIC intron Domain 1, 2 & 3

Group IIC Intron Domain 1, 2 & 3 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1, 2 & 3 monomer, 103 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
RgGuinier 3.6 nm
Dmax 12.0 nm
VolumePorod 195 nm3

SASDX69 – Group IIC intron Domain 1, 2. 3 & 4

Group IIC intron Domain 1, 2, 3 & 4 experimental SAS data
DAMMIF model
Sample: Group IIC intron Domain 1, 2, 3 & 4 monomer, 125 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
RgGuinier 4.2 nm
Dmax 12.6 nm
VolumePorod 247 nm3