A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase.

Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE
Nucleic Acids Res 54(2) (2026 Jan 14)
PMID: 41556341
doi: 10.1093/nar/gkag029 		Submitted to SASBDB: 2025 Sep 18
Published in SASBDB:

SASDXB9 – O’Nyong-Nyong virus (ONNV) nsP4 full length wild type

RNA-directed RNA polymerase nsP4 experimental SAS data
RNA-directed RNA polymerase nsP4 Kratky plot
Sample: RNA-directed RNA polymerase nsP4 monomer, 68 kDa O'nyong-nyong virus protein
Buffer: 25 mM HEPES, 1 mM TCEP, 5% glycerol and 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jun 8
RgGuinier 3.6 nm

SASDXC9 – O'Nyong Nyong virus C-terminus-50residues-nsP3-nsP4 fusion protein

50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 experimental SAS data
50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 Kratky plot
Sample: 50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 tetramer, 318 kDa O'nyong nyong virus protein
Buffer: 25 mM HEPES 5% glycerol, 1 mM TCEP, 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2025 Aug 14
RgGuinier 5.2 nm