Prothrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers.

Troisi R, Cangiano A, Cowieson N, Spiridonova V, Pozzi N, Del Vecchio P, Paduano L, Sica F
Mol Ther (2026 Jul 23)
PMID: 42494063
doi: 10.1016/j.ymthe.2026.07.042 		Submitted to SASBDB: 2025 Nov 8
Published in SASBDB:

SASDYL2 – Human prothrombin in the unbound state

Human prothrombin experimental SAS data
MULTIFOXS model
Sample: Human prothrombin monomer, 72 kDa Homo sapiens protein
Buffer: 10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 29
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 123 nm3

SASDYM2 – Human prothrombin complex with the oligonucleotide aptamer NU172

Human prothrombinNU172 experimental SAS data
MULTIFOXS model
Sample: Human prothrombin monomer, 72 kDa Homo sapiens protein
NU172 monomer, 8 kDa DNA
Buffer: 10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 29
RgGuinier 3.9 nm
Dmax 13.8 nm
VolumePorod 124 nm3