Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System.

Bunney TD, Inglis AJ, Sanfelice D, Farrell B, Kerr CJ, Thompson GS, Masson GR, Thiyagarajan N, Svergun DI, Williams RL, Breeze AL, Katan M
Structure 26(3):446-458.e8 (2018 Mar 6)

PMID: 29478821 doi: 10.1016/j.str.2018.01.016

Submitted to SASBDB: 2017 Jan 18 Published in SASBDB: 2018 Mar 20

SASDBP9 – Human Hsp90 co-chaperone Cdc37 protein (CD37) Sample: Hsp90 co-chaperone Cdc37 monomer, 44 kDa Homo sapiens protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10 RgGuinier 4.0 nm Dmax 15.5 nm VolumePorod 106 nm3

SASDBQ9 – Human Hsp90 co-chaperone Cdc37 (CD37) in complex with fibroblast growth factor receptor 3 Sample: Hsp90 co-chaperone Cdc37 monomer, 44 kDa Homo sapiens protein Fibroblast growth factor receptor 3 monomer, 35 kDa Homo sapiens protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10 RgGuinier 4.7 nm Dmax 19.5 nm VolumePorod 161 nm3

SASDBR9 – Human fibroblast growth factor receptor 3, from SEC-SAXS Sample: Fibroblast growth factor receptor 3 monomer, 35 kDa Homo sapiens protein Buffer: 25 mM Tris.Cl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10 RgGuinier 2.4 nm Dmax 8.3 nm VolumePorod 59 nm3