The Rho guanine-nucleotide exchange factor P-Rex2 exhibits structural and regulatory features distinct from the related RhoGEF P-Rex1

Anderson L, Marde R, Muma G, Nayak V, Phan C, Li S, Cash J
Journal of Biological Chemistry 302(7):113229 (2026 Jul)

doi: 10.1016/j.jbc.2026.113229 		Submitted to SASBDB: 2026 Apr 3
Published in SASBDB:

SASDZU5 – Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein DH/PH domain and DEP1 module (P-Rex2 DH/PH-DEP1)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH-DEP1 experimental SAS data
BILBOMD model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH-DEP1 monomer, 55 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2025 Jun 10
RgGuinier 2.9 nm
Dmax 10.5 nm
VolumePorod 77 nm3

SASDZV5 – Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger protein 2 DH/PH domain tandem (P-Rex2 DH/PH)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem experimental SAS data
BILBOMD model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2025 Jun 10
RgGuinier 2.6 nm
Dmax 8.4 nm
VolumePorod 52 nm3