Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.

Zúñiga-Domínguez JA, Jain R, González-Andrade M, Farquhar ER, Chance MR, Gijsbers A, Sánchez-Puig N
Protein Sci 35(4):e70504 (2026 Apr)
PMID: 41848393
doi: 10.1002/pro.70504 		Submitted to SASBDB: 2025 Dec 22
Published in SASBDB:

SASDYG4 – Yeast Elongation Factor like-1 protein (EFL1)

Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) experimental SAS data
Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) Kratky plot
Sample: Ribosome assembly protein 1 with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) monomer, 127 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3
RgGuinier 4.6 nm
Dmax 15.7 nm
VolumePorod 368 nm3

SASDYF4 – Mutant Elongation Factor Like-1 protein

Mutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) experimental SAS data
Mutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) Kratky plot
Sample: Mutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH) monomer, 127 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3
RgGuinier 4.7 nm
Dmax 15.2 nm
VolumePorod 314 nm3