Biochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.

Sirigu S, Roret T, Mocaër PY, Larocque R, Jouanneau D, Legrand P, Baudoux AC, Czjzek M
Acta Crystallogr D Struct Biol (2026 Jul 1)

PMID: 42308020 doi: 10.1107/S2059798326005425

Submitted to SASBDB: 2026 Feb 14 Published in SASBDB: 2026 Jun 20

SASDYX6 – trimeric depolymerase Dpo31 lacking domain D1, mature form without D4 Sample: Tailspike protein depolymerase Dpo31 trimer, 203 kDa Cobetia phage Carin1 protein Buffer: Tris-HCl 50 mM pH 8, NaCl 100 mM, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 14 RgGuinier 5.6 nm Dmax 19.0 nm VolumePorod 207 nm3

SASDYY6 – trimeric chaperon domain D4 of depolymerase Dpo31 Sample: Chaperon for trimerisation of Dpo31 trimer, 54 kDa Cobetia phage Carin1 protein Buffer: Tris-HCl 50 mM pH 8, NaCl 100 mM, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 14 RgGuinier 2.5 nm Dmax 6.4 nm VolumePorod 66 nm3

SASDYZ6 – trimeric tailspike protein depolymerase Dpo31, mature form without D4 Sample: Tailspike protein depolymerase Dpo31 trimer, 203 kDa Cobetia phage Carin1 protein Buffer: Tris-HCl 50 mM pH 8, NaCl 100 mM, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 10 RgGuinier 7.0 nm Dmax 28.6 nm VolumePorod 307 nm3