FHL2 production enabled new insight into its flexibility and unexpected binding stoichiometry with β-catenin.

Logonder T, Prešern U, Gaber A
J Struct Biol X 14:100159 (2026 Dec)

PMID: 42668614 doi: 10.1016/j.yjsbx.2026.100159

Submitted to SASBDB: 2026 Apr 21 Published in SASBDB: 2026 Sep 2

SASDZ36 – Full-length tag-free Four and a half LIM domains protein 2 (FHL2), 7.5 mg/ml Sample: Four and a half LIM domains protein 2 monomer, 32 kDa Escherichia coli protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 % (v/v) glycerol, 10 mM β-mercaptoethanol, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Jul 10 RgGuinier 3.8 nm Dmax 13.7 nm VolumePorod 43 nm3