A chimeric, half-life extended lysin with a unique mode of action

Visram Z, Kestemont D, Bauer B, Qiao R, Durica-Mitic S, Majoros-Hashempour A, Schmidt J, Berdaguer R, Krey K, Mutti M, Zerbs M, von Freyberg M, Corsini L, Badarau A
Frontiers in Microbiology 17 (2026 Aug 21)

doi: 10.3389/fmicb.2026.1880134

Submitted to SASBDB: 2026 May 13 Published in SASBDB: 2026 Jul 23

SASDZ88 – Synthetic fusion protein comprising human IgG1 Fc, the CHAP domain of engineered Staphylococcus aureus LytN, and engineered Staphylococcus simulans lysostaphin (Fc-L2a-CHAP(alt)-LSN(HEK)) Sample: Fc-L2a-CHAP(alt)-LSN(HEK) dimer, 156 kDa synthetic construct protein Buffer: 20 mM HEPES, 150 mM NaCl, pH 7.0, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Apr 6 RgGuinier 8.9 nm Dmax 40.0 nm

SASDZ98 – Engineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK)) Sample: Fc-L1-CHAP(opt)-LSN(HEK) dimer, 148 kDa synthetic construct protein Buffer: 20 mM HEPES, 150 mM NaCl, pH 7.0, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Apr 6 RgGuinier 7.3 nm Dmax 40.0 nm