Intramolecular Interactions between Folded and Disordered Regions Shape Ubiquilin Structure and Function

Niblo J, Acharya N, Watkins M, Castañeda C, Sukenik S
Advanced Science (2026 Jun 02)

doi: 10.1002/advs.75904 		Submitted to SASBDB: 2026 May 29
Published in SASBDB:

SASDZ99 – Full-length yeast shuttle protein Dsk2 (200 ul injection)

Ubiquitin domain-containing protein DSK2 experimental SAS data
Ubiquitin domain-containing protein DSK2 Kratky plot
Sample: Ubiquitin domain-containing protein DSK2 monomer, 39 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Feb 13
RgGuinier 3.7 nm

SASDZA9 – Full-length yeast shuttle protein Dsk2 (300 ul injection volume)

Ubiquitin domain-containing protein DSK2 experimental SAS data
Ubiquitin domain-containing protein DSK2 Kratky plot
Sample: Ubiquitin domain-containing protein DSK2 monomer, 39 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 7
RgGuinier 3.8 nm

SASDZB9 – Full-length yeast shuttle protein Dsk2 with single point mutation (I45A)

Ubiquitin domain-containing protein DSK2 I45A experimental SAS data
Ubiquitin domain-containing protein DSK2 I45A Kratky plot
Sample: Ubiquitin domain-containing protein DSK2 I45A monomer, 39 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Feb 13
RgGuinier 3.9 nm

SASDZC9 – Full-length yeast shuttle protein Dsk2 with HS1 deletion (residues 114-134)

DSK2 with HS1 deletion experimental SAS data
DSK2 with HS1 deletion Kratky plot
Sample: DSK2 with HS1 deletion monomer, 37 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Feb 13
RgGuinier 3.6 nm

SASDZD9 – Full-length yeast shuttle protein Dsk2 with HS2 deletion (residues 279-291)

DSK2 with HS2 deletion experimental SAS data
DSK2 with HS2 deletion Kratky plot
Sample: DSK2 with HS2 deletion monomer, 38 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Feb 13
RgGuinier 3.9 nm

SASDZE9 – Full-length yeast shuttle protein Dsk2 with HS3 deletion (residues 303-313)

DSK2 with HS3 deletion experimental SAS data
DSK2 with HS3 deletion Kratky plot
Sample: DSK2 with HS3 deletion monomer, 38 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 7
RgGuinier 4.1 nm