Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica

Christopher E Ruth
University of Arkansas Dissertation - (2018 December)

URL: https://www.proquest.com/openview/18948954fcfd8d46315cff1b306c60c4/

Submitted to SASBDB: 2017 Jun 2 Published in SASBDB: 2019 Aug 7

SASDCS4 – Collagenase ColG s2s3as3b at pCa 3 Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.0 nm Dmax 12.7 nm VolumePorod 52 nm3

SASDD63 – Collagenase ColG s2s3as3b at pCa 3 (second analysis) Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 11 RgGuinier 3.0 nm Dmax 14.4 nm VolumePorod 61 nm3

SASDCT4 – Collagenase ColG s2s3as3b at pCa 4 Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.3 nm Dmax 11.5 nm VolumePorod 63 nm3

SASDCU4 – Collagenase ColG s2s3as3b at pCa 5 Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 4.3 nm Dmax 19.7 nm VolumePorod 98 nm3

SASDCV4 – Collagenase ColG s2s3as3b at pCa 6 Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.6 nm Dmax 15.2 nm VolumePorod 41 nm3

SASDCN4 – Collagenase ColH s2as2bs3 at pCa 3 Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.3 nm Dmax 13.6 nm VolumePorod 35 nm3

SASDCP4 – Collagenase ColH s2as2bs3 at pCa 4 Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.2 nm Dmax 13.1 nm VolumePorod 37 nm3

SASDCQ4 – Collagenase ColH s2as2bs3 at pCa 5 Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.3 nm Dmax 14.2 nm VolumePorod 37 nm3

SASDCR4 – Collagenase ColH s2as2bs3 at pCa 6 Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.2 nm Dmax 15.5 nm VolumePorod 35 nm3

SASDC35 – Collagenase ColH s2as2bs3 at pCa 7 Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.0 nm Dmax 14.4 nm VolumePorod 40 nm3