The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis.

Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q
Biochem J 475(7):1295-1308 (2018 Apr 9)

PMID: 29555845 doi: 10.1042/BCJ20180079

Submitted to SASBDB: 2017 Nov 23 Published in SASBDB: 2018 Sep 5

SASDCH9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) with GDP Sample: Sensory box/response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26 RgGuinier 5.0 nm Dmax 20.0 nm VolumePorod 299 nm3

SASDCJ9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP Sample: Sensory box/response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5 Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26 RgGuinier 4.8 nm Dmax 17.0 nm VolumePorod 271 nm3