The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity.

Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
J Biol Chem (2019 Jul 10)

PMID: 31292194 doi: 10.1074/jbc.RA119.007393

Submitted to SASBDB: 2019 Jul 26 Published in SASBDB: 2019 Oct 31

SASDGP2 – Thymine dioxygenase full length J-DNA binding protein (JBP1) Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21 RgGuinier 3.4 nm Dmax 12.0 nm VolumePorod 128 nm3

SASDGQ2 – Thymine dioxygenase lacking the JDNA binding domain Delta-JDBD (Δ-JDBD) Sample: Delta-JDBD monomer, 72 kDa Leismania tarentolae protein Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21 RgGuinier 3.1 nm Dmax 9.9 nm VolumePorod 108 nm3

SASDGR2 – Thymine dioxygenase J-containing DNA binding domain (JDBD) Sample: J-DNA binding domain monomer, 21 kDa Leishmania tarentolae protein Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 4 RgGuinier 2.2 nm Dmax 7.1 nm VolumePorod 38 nm3

SASDGS2 – J-23-DNA Sample: J-DNA (23mer) monomer, 14 kDa DNA Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21 RgGuinier 2.3 nm Dmax 7.3 nm VolumePorod 20 nm3

SASDGT2 – Thymine dioxygenase J-containing DNA binding domain in complex with J-23-DNA (JDBD:J-23-DNA) Sample: J-DNA binding domain monomer, 21 kDa Leishmania tarentolae protein J-DNA (23mer) monomer, 14 kDa DNA Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21 RgGuinier 2.5 nm Dmax 8.6 nm VolumePorod 43 nm3

SASDGU2 – Thymine dioxygenase full length J-DNA binding protein in complex with J-23-DNA (JBP1:J-23-DNA) Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein J-DNA (23mer) monomer, 14 kDa DNA Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21 RgGuinier 4.1 nm Dmax 14.1 nm VolumePorod 148 nm3