Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.

Chicano A, Crosas E, Otón J, Melero R, Engel BD, Daban JR
EMBO J 38(7) (2019 Apr 1)

PMID: 30609992 doi: 10.15252/embj.201899769

Submitted to SASBDB: 2018 Mar 30 Published in SASBDB: 2023 Dec 7

SASDDQ5 – Human metaphase chromosomes in 17mM magnesium chloride Sample: Metaphase chromosomes in 17 mM magnesium chloride monomer, 0 kDa Homo sapiens Buffer: 10 mM PIPES, 17 mM magnesium chloride, 20 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 May 23

SASDDR5 – Human metaphase chromosomes in 5 mM magnesium chloride Sample: Human metaphase chromosomes in 5 mM magnesium chloride monomer, 0 kDa Homo sapiens Buffer: 10 mM PIPES, 5 mM magnesium chloride, 10 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Jun 6

SASDDS5 – Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride Sample: Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride monomer, 0 kDa Homo sapiens Buffer: 10 mM PIPES, hexaamminecobalt(III) chloride, 40% glycerol, pH: 7.2 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Sep 12