Structural interpretations of a flexible cold-active AMS8 lipase by combining small-angle X-ray scattering and molecular dynamics simulation (SAXS-MD).

Yaacob N, Kamonsutthipaijit N, Soontaranon S, Leow TC, Rahman RNZRA, Ali MSM
Int J Biol Macromol (2022 Aug 24)
PMID: 36029961
doi: 10.1016/j.ijbiomac.2022.08.145 		Submitted to SASBDB: 2018 Oct 27
Published in SASBDB:

SASDEX6 – Cold-active AMS8 lipase in the presence of calcium (without polyhistidine affinity tag)

LipAMS8 experimental SAS data
DAMMIF model
Sample: LipAMS8 monomer, 50 kDa Pseudomonas sp. AMS8 protein
Buffer: 50 mM Tris HCl, 5 mM CaCl2, pH: 8
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2018 Mar 6
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 87 nm3