Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides.

Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N
Proc Natl Acad Sci U S A 116(3):835-844 (2019 Jan 15)

PMID: 30606802 doi: 10.1073/pnas.1813450116

Submitted to SASBDB: 2018 May 14 Published in SASBDB: 2019 Jan 4

SASDDJ6 – SAM-dependent O-Methyltransferase from Mycobacterium hassiacum Sample: Methyltransferase domain protein dimer, 51 kDa Mycolicibacterium hassiacum protein Buffer: 20 mM bis-tris propane, 50 mM NaCl, 2 mM DTT, 5% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jun 21 RgGuinier 2.6 nm Dmax 8.5 nm VolumePorod 86 nm3