Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.

Lorenz C, Ince S, Zhang T, Cousin A, Batra-Safferling R, Nagel-Steger L, Herrmann C, Stadler AM
FEBS J (2019 Jul 22)

PMID: 31330084 doi: 10.1111/febs.15015

Submitted to SASBDB: 2018 Nov 27 Published in SASBDB: 2019 Jul 24

SASDEE8 – Farnesylated human Guanylate Binding Protein 1 (farn-hGBP1), monomer from SEC-SAXS Sample: Farnesylated human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30 RgGuinier 3.9 nm Dmax 13.0 nm VolumePorod 102 nm3

SASDEF8 – Human Guanylate Binding Protein 1 (hGBP1), monomer from SEC-SAXS Sample: Human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30 RgGuinier 3.9 nm Dmax 13.4 nm VolumePorod 106 nm3

SASDEG8 – Human Guanylate Binding Protein 1 with GppNHp (hGBP1 + GppNHp), dimer from SEC-SAXS Sample: Human Guanylate-binding protein 1 dimer, 138 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30 RgGuinier 5.5 nm Dmax 22.0 nm VolumePorod 270 nm3

SASDEH8 – Human Guanylate Binding Protein 1 with GppNHp (hGBP1 + GppNHp), monomer from SEC-SAXS Sample: Human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30 RgGuinier 4.5 nm Dmax 20.5 nm VolumePorod 120 nm3

SASDEJ8 – Human Guanylate Binding Protein 1 (hGBP1), dimer from SEC-SAXS Sample: Human Guanylate-binding protein 1 dimer, 138 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30 RgGuinier 4.8 nm Dmax 15.6 nm VolumePorod 211 nm3

SASDEK8 – Farnesylated human Guanylate Binding Protein 1 (farn-hGBP1), batch mode Sample: Farnesylated human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2016 May 8 RgGuinier 3.8 nm Dmax 14.3 nm VolumePorod 102 nm3

SASDEL8 – Human Guanylate Binding Protein 1 (hGBP1), batch mode Sample: Human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2016 May 8 RgGuinier 5.1 nm Dmax 27.4 nm VolumePorod 162 nm3

SASDEM8 – Human Guanylate Binding Protein 1 with GppNHp (hGBP1 + GppNHp), batch mode Sample: Human Guanylate-binding protein 1 monomer, 69 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp, pH: 7.9 Experiment: SAXS data collected at BM29, ESRF on 2016 May 8 RgGuinier 5.5 nm Dmax 27.0 nm VolumePorod 186 nm3