Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth

Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
(2018 Nov 06)

doi: 10.1101/464131 		Submitted to SASBDB: 2018 Oct 16
Published in SASBDB:

SASDEF6 – Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455)

Epstein-Barr nuclear antigen 2 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
RgGuinier 2.7 nm
Dmax 9.3 nm
VolumePorod 19 nm3

SASDEG6 – Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)

Epstein-Barr nuclear antigen 2 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
RgGuinier 2.8 nm
Dmax 10.4 nm
VolumePorod 20 nm3

SASDEH6 – BS69 (CC-MYND: Zinc finger MYND domain-containing protein 11, amino acids 480-602)

Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Zinc finger MYND domain-containing protein 11 tetramer, 60 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
RgGuinier 5.3 nm
Dmax 16.9 nm
VolumePorod 83 nm3

SASDEJ6 – Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)

Epstein-Barr nuclear antigen 2Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Zinc finger MYND domain-containing protein 11 tetramer, 60 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
RgGuinier 4.6 nm
Dmax 13.8 nm
VolumePorod 72 nm3

SASDEK6 – Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)

Epstein-Barr nuclear antigen 2Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Zinc finger MYND domain-containing protein 11 hexamer, 90 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
RgGuinier 4.7 nm
Dmax 14.5 nm
VolumePorod 239 nm3