Molecular Fingerprints for a Novel Enzyme Family in Actinobacteria with Glucosamine Kinase Activity.

Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB
MBio 10(3) (2019 May 14)

PMID: 31088917 doi: 10.1128/mBio.00239-19

Submitted to SASBDB: 2018 Oct 15 Published in SASBDB: 2019 May 9

SASDEL6 – Glucosamine kinase from Streptacidiphilus jiangxiensis Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24 RgGuinier 2.6 nm Dmax 8.0 nm VolumePorod 72 nm3

SASDEM6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 0.2 M D-glucosamine, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 20 RgGuinier 2.6 nm Dmax 8.0 nm VolumePorod 78 nm3

SASDEN6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 1 mM ATP Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 1 mM ATP, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24 RgGuinier 2.5 nm Dmax 7.8 nm VolumePorod 73 nm3

SASDEP6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine and 1 mM ATP Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 0.2 M D-glucosamine, 1 mM ATP, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 20 RgGuinier 2.5 nm Dmax 7.7 nm VolumePorod 76 nm3

SASDEQ6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 50 mM D-glucose Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 50 mM D-glucose, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2018 Apr 13 RgGuinier 2.6 nm Dmax 8.0 nm VolumePorod 73 nm3

SASDER6 – Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 50 mM D-glucose and 1 mM ATP Sample: Glucosamine kinase monomer, 48 kDa Streptacidiphilus jiangxiensis protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 50 mM D-glucose, 1 mM ATP, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24 RgGuinier 2.5 nm Dmax 7.7 nm VolumePorod 73 nm3