Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic beta-oxidation trifunctional enzyme complexes.

Sah-Teli SK, Hynönen MJ, Schmitz W, Geraets JA, Seitsonen J, Pedersen JS, Butcher SJ, Wierenga RK, Venkatesan R
Biochem J (2019 Jun 25)
PMID: 31235482
doi: 10.1042/BCJ20190314
Submitted to SASBDB: 2018 Dec 28
Published in SASBDB:

SASDEL9 – Escherichia coli aerobic fatty acid beta-oxidation trifunctional enzyme complex

Fatty acid oxidation complex subunit alphaFatty acid oxidation complex subunit alpha3-ketoacyl-CoA thiolase FadA (beta subunit) experimental SAS data
DAMMIN model
Sample: Fatty acid oxidation complex subunit alpha monomer, 81 kDa Escherichia coli (strain ... protein
Fatty acid oxidation complex subunit alpha monomer, 81 kDa Escherichia coli (strain ... protein
3-ketoacyl-CoA thiolase FadA (beta subunit) dimer, 82 kDa Escherichia coli (strain ... protein
Buffer: 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 120 mM KCl, 2.5 mM DTT, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 30
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 406 nm3

SASDEM9 – anEcTFE: Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme complex

anaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic 3-ketoacyl-CoA thiolase FadI beta subunitanaerobic 3-ketoacyl-CoA thiolase FadI beta subunit experimental SAS data
DAMMIN model
Sample: Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain ... protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain ... protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain ... protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain ... protein
Anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli (strain ... protein
Anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli (strain ... protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Sep 22
RgGuinier 6.2 nm
Dmax 19.6 nm
VolumePorod 856 nm3