Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein.

Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
Nat Commun 6:7271 (2015 Jun 1)

PMID: 26027519 doi: 10.1038/ncomms8271

Submitted to SASBDB: 2014 Nov 27 Published in SASBDB: 2015 Jul 7

SASDA37 – Surface Protein G (SasG) EG5 repeat protein G51-G52 Sample: Surface protein G monomer, 24 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 4.7 nm Dmax 19.0 nm VolumePorod 29 nm3

SASDA47 – Surface Protein G (SasG) EG5 repeat protein G51-G53 Sample: Surface protein G monomer, 39 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 7.7 nm Dmax 30.5 nm VolumePorod 49 nm3

SASDA57 – Surface Protein G (SasG) EG5 repeat protein G51-G54 Sample: Surface protein G monomer, 53 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 9.7 nm Dmax 38.5 nm VolumePorod 58 nm3

SASDA67 – Surface Protein G (SasG) EG5 repeat protein G51-G55 Sample: Surface protein G monomer, 67 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 12.0 nm Dmax 48.0 nm VolumePorod 87 nm3

SASDA77 – Surface Protein G (SasG) EG5 repeat protein G51-G56 Sample: Surface protein G monomer, 81 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 14.1 nm Dmax 57.0 nm VolumePorod 89 nm3

SASDA87 – Surface Protein G (SasG) EG5 repeat protein G51-G57 Sample: Surface protein G monomer, 95 kDa Staphylococcus aureus protein Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12 RgGuinier 15.9 nm Dmax 63.0 nm VolumePorod 122 nm3