Distinctive features and structural significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site

Al Kikhney, Marco Salomone-Stagni

Submitted to SASBDB: 2015 Feb 17 Published in SASBDB: 2017 Feb 8

SASDAF7 – Metal-bound hETHE1 Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 21 RgGuinier 2.5 nm Dmax 7.5 nm VolumePorod 70 nm3

SASDAH7 – Metal-free hETHE1, 0.5 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 9.0 nm Dmax 32.3 nm VolumePorod 400 nm3

SASDAJ7 – Metal-free hETHE1, 1 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 8.3 nm Dmax 38.0 nm VolumePorod 602 nm3

SASDAK7 – Metal-free hETHE1, 2 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 8.8 nm Dmax 42.5 nm VolumePorod 647 nm3

SASDAL7 – Metal-free hETHE1, 3 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 11.2 nm Dmax 56.0 nm VolumePorod 1445 nm3

SASDAM7 – Metal-free hETHE1, 5 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 12.4 nm Dmax 59.0 nm VolumePorod 1820 nm3

SASDAN7 – Metal-free hETHE1, 10 mg/ml Sample: Persulfide dioxygenase ETHE1, mitochondrial dimer, 56 kDa Homo sapiens protein Buffer: 50 mM Tris 150 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 23 RgGuinier 14.2 nm Dmax 63.5 nm VolumePorod 2317 nm3