Conformational flexibility of fork-remodeling helicase Rad5 shown by full-ensemble hybrid methods.

Gildenberg MS, Washington MT
PLoS One 14(10):e0223875 (2019)

PMID: 31626633 doi: 10.1371/journal.pone.0223875

Submitted to SASBDB: 2019 Aug 29 Published in SASBDB: 2019 Oct 8

SASDG25 – DNA repair protein Rad5 (with 6xhis and twin-strep tags) Sample: DNA repair protein RAD5 monomer, 138 kDa Saccharomyces cerevisiae protein Buffer: 40 mM Tris, 150 mM KCl, 5 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 6 RgGuinier 4.7 nm Dmax 17.8 nm VolumePorod 254 nm3