Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes

David Lambright Submitted to SASBDB: 2019 Aug 17
Published in SASBDB:

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG94 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDGA4 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 194 nm3

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.3 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGB4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDGC4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Cytohesin-3 experimental SAS data
CORAL model
Sample: Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.1 nm
Dmax 25.7 nm
VolumePorod 168 nm3

SASDG84 – Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)

Cytohesin-2 experimental SAS data
DAMMIF model
Sample: Cytohesin-2 dimer, 95 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
RgGuinier 5.3 nm
Dmax 27.0 nm
VolumePorod 180 nm3