The structural basis of fungal glucuronoyl esterase activity on natural substrates.

Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S
Nat Commun 11(1):1026 (2020 Feb 24)
PMID: 32094331
doi: 10.1038/s41467-020-14833-9
Submitted to SASBDB: 2019 Sep 27
Published in SASBDB:

SASDGC6 – Glucuronoyl esterase, deglycolsylated full length

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) experimental SAS data
DAMMIN model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) monomer, 51 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 71 nm3

SASDGD6 – Glucuronoyl esterase S286A, deglycolsylated truncated (amino acids 95-474)

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) experimental SAS data
PDB model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) monomer, 43 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
RgGuinier 2.0 nm
Dmax 6.1 nm
VolumePorod 50 nm3