Structural Insights on Dynamic behavior of PDI in solution environment through SAXS technique

Chandra Sekar Submitted to SASBDB: 2019 Oct 17
Published in SASBDB:

SASDGZ9 – Protein Disulfide Isomerase (PDI) purified from bovine liver

Protein disulfide-isomerase experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 98 nm3

SASDH22 – Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI)

Protein disulfide-isomerasePancreatic trypsin inhibitor experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 7 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
RgGuinier 3.2 nm
Dmax 8.9 nm
VolumePorod 88 nm3

SASDH32 – Protein Disulfide Isomerase (PDI) in Complex with Ribonuclease-A (RNase)

Protein disulfide-isomeraseRibonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
RgGuinier 3.0 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDH42 – Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5

Pancreatic trypsin inhibitor experimental SAS data
DAMMIF model
Sample: Pancreatic trypsin inhibitor dimer, 13 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 6 nm3

SASDH52 – Ribonuclease A (Type III-A) in a solution environment at pH 7.5

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 20 nm3