Structural Insights on Dynamic behavior of PDI in solution environment through SAXS technique

Chandra Sekar

Submitted to SASBDB: 2019 Oct 17 Published in SASBDB: 2021 Sep 20

SASDGZ9 – Protein Disulfide Isomerase (PDI) purified from bovine liver Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4 RgGuinier 3.0 nm Dmax 9.8 nm VolumePorod 98 nm3

SASDH22 – Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI) Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 7 kDa Bos taurus protein Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4 RgGuinier 3.2 nm Dmax 8.9 nm VolumePorod 88 nm3

SASDH32 – Protein Disulfide Isomerase (PDI) in Complex with Ribonuclease-A (RNase) Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4 RgGuinier 3.0 nm Dmax 9.1 nm VolumePorod 85 nm3

SASDH42 – Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5 Sample: Pancreatic trypsin inhibitor dimer, 13 kDa Bos taurus protein Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4 RgGuinier 1.4 nm Dmax 4.6 nm VolumePorod 6 nm3

SASDH52 – Ribonuclease A (Type III-A) in a solution environment at pH 7.5 Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4 RgGuinier 1.9 nm Dmax 7.2 nm VolumePorod 20 nm3