Structural analyses of the group A flavin-dependent monooxygenase PieE reveal a sliding FAD cofactor conformation bridging OUT and IN conformations.

Manenda MS, Picard MÈ, Zhang L, Cyr N, Zhu X, Barma J, Pascal JM, Couture M, Zhang C, Shi R
J Biol Chem (2020 Feb 28)
PMID: 32111738
doi: 10.1074/jbc.RA119.011212
Submitted to SASBDB: 2019 Oct 30
Published in SASBDB:

SASDHC2 – PieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin adenine dinucleotide

2,4-dichlorophenol 6-monooxygenaseFlavin adenine dinucleotide experimental SAS data
DAMMIN model
Sample: 2,4-dichlorophenol 6-monooxygenase hexamer, 399 kDa Streptomyces sp. SCSIO … protein
Flavin adenine dinucleotide hexamer, 5 kDa
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM DTT, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2019 Oct 22
RgGuinier 4.8 nm
Dmax 13.2 nm
VolumePorod 624 nm3