Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor.

Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
Commun Biol 3(1):498 (2020 Sep 9)

PMID: 32908216 doi: 10.1038/s42003-020-01211-w

Submitted to SASBDB: 2019 Dec 30 Published in SASBDB: 2020 May 11

SASDHL4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Oct 12 RgGuinier 3.7 nm Dmax 10.1 nm VolumePorod 140 nm3

SASDHM4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) from SEC-SAXS Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12 RgGuinier 3.7 nm Dmax 13.5 nm VolumePorod 147 nm3

SASDHN4 – N-terminal 5 domains of the cation-independent mannose-6-phosphate receptor (CI-MPR) bound to mannose 6-phosphate (M6P) Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12 RgGuinier 3.7 nm Dmax 13.3 nm VolumePorod 150 nm3

SASDHP4 – Palmitoyl-protein thioesterase 1 (PPT1) Sample: Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12 RgGuinier 2.3 nm Dmax 9.8 nm VolumePorod 54 nm3

SASDHQ4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) in complex with palmitoyl-protein thioesterase 1 (PPT1) Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12 RgGuinier 4.9 nm Dmax 19.3 nm VolumePorod 258 nm3