Direct localization of detergents and bacteriorhodopsin in the lipidic cubic phase by small-angle neutron scattering

Cleveland IV T, Blick E, Krueger S, Leung A, Darwish T, Butler P
IUCrJ 8(1) (2021 Jan 01)

doi: 10.1107/S2052252520013974
Submitted to SASBDB: 2020 Jun 12
Published in SASBDB:

SASDJD4 – Bacteriorhodopsin solubilized in contrast-matched octyl glucoside

Bacteriorhodopsin experimental SAS data
Bacteriorhodopsin Kratky plot
Sample: Bacteriorhodopsin monomer, 27 kDa Halobacterium salinarum protein
Buffer: 25 mM NaH2PO4, 1.35 mM KOH, 40 mM partially-deuterated octyl glucoside mixture, pH: 5.6
Experiment: SANS data collected at NGB30, NIST Center for Neutron Research on 2017 Jan 20
RgGuinier 2.7 nm
Dmax 5.4 nm
VolumePorod 68 nm3

SASDJE4 – Bacteriorhodopsin in contrast-matched lipidic cubic phase formed with deuterated monoolein

Bacteriorhodopsin experimental SAS data
Bacteriorhodopsin Kratky plot
Sample: Bacteriorhodopsin monomer, 27 kDa Halobacterium salinarum protein
Buffer: 25 mM NaH2PO4, 1.35 mM KOH, 40 mM octyl glucoside, pH: 5.6
Experiment: SANS data collected at NG7, NIST Center for Neutron Research on 2017 May 15
RgGuinier 2.7 nm
Dmax 6.2 nm
VolumePorod 49 nm3