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77 hits found for Cy M Jeffries

SASDC22 – ...m LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7

Homeobox protein CEH-14CeLIM-7 experimental SAS data
DAMMIN model
Sample: ...meobox protein CEH-14 monomer, 16 kDa Caenorhabditis elegans protein
...M-7 monomer, 4 kDa Caenorhabditis elegans protein
Buffer: ...mM Tris, 100 mM NaCl, 5 mM TCEP, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSess, University of Sydney on 2009 Apr 7
...mily LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans. Sci Rep 7(1):4579 (2017)
...M, Llamosas E, Jacques DA, Jeffries CM, Dastmalchi S, Ripin N, Nicholas HR, Matthews JM
RgGuinier 2.4 nm
Dmax 8.9 nm
VolumePorod 26 nm3

SASDD32 – ...methyltransferase from Acinetobacter baumannii ATCC 17978

DNA-(adenine N6)-methyltransferase experimental SAS data
DAMMIN model
Sample: ...methyltransferase monomer, 49 kDa ...mannii protein
Buffer: ...mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2016 Sep 30
...mbinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity. Protein Expr Purif 151:78-85 (2018)
...Jeffries CM, Svergun DI, Wilharm G
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 96 nm3

SASDD42 – ...cystis fluorescence recovery protein SynFRP.8-109

Fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Fluorescence recovery protein ...mer, 23 kDa ...cystis sp. PCC … protein
Buffer: ...mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Sep 1
...mology FRPs from different cyanobacteria with Synechocystis OCP. ...m Biophys Acta 1859(5):382-393 (2018)
...mskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 36 nm3

SASDD52 – ...maxFRP.1-106

Uncharacterized fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Uncharacterized fluorescence recovery protein ...mer, 24 kDa ...maxima CS-328 protein
Buffer: ...mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Sep 1
...mology FRPs from different cyanobacteria with Synechocystis OCP. ...m Biophys Acta 1859(5):382-393 (2018)
...mskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDH92 – ...modium falciparum lipocalin (PF3D7_0925900): Dimer-tetramer equilibrium through a concentration series (combined batch and SEC-SAXS measurements)

Plasmodium falciparum Lipocalin experimental SAS data
SASREF MX model
Sample: ...modium falciparum Lipocalin ...mer, 89 kDa ...modium falciparum protein
Buffer: ...mM Tris pH7.5, 150 mM NaCl, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 8
...Malaria Parasite Plasmodium falciparum Cell Reports 31(12):107817 (2020)
...Jeffries C, Svergun D, Wilson D, Wilmanns M, Gilberger T
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 126 nm3

SASDAB2 – Cytochrome c from equine heart

Cytochrome cHeme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c monomer, 12 kDa Equus caballus protein
...me C monomer, 1 kDa
Buffer: ...mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2018 Apr 8
Standard proteins
Cy M Jeffries
RgGuinier 1.3 nm
Dmax 3.7 nm
VolumePorod 12 nm3

SASDBJ3 – ...m albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...m albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: ...mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2014 Jan 23
...monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDDJ3 – Candida antarctica lipase B - with guanidine-HCl unfolding series

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: ...m Pseudozyma antarctica , 33 kDa Moesziomyces antarcticus protein
Buffer: ...mM NaCl, 20 mM Na2HPO4, pH: 6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Jeffries CM, Svergun DI
RgGuinier 2.4 nm

SASDBK3 – ...m albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...m albumin, dimer ...mer, 133 kDa Bos taurus protein
Buffer: ...mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2014 Jan 23
...monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDDK3 – Candida antarctica lipase B - with guanidine-HCl unfolding series, in the presence of dithiothreitol

Lipase B from Pseudozyma antarctica experimental SAS data
Lipase B from Pseudozyma antarctica Kratky plot
Sample: ...m Pseudozyma antarctica , 33 kDa Moesziomyces antarcticus protein
Buffer: ...mM NaCl, 20 mM Na2HPO4, 10 mM DTT, pH: 6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Jeffries CM, Svergun DI
RgGuinier 2.4 nm

SASDDL3 – Folded ribonuclease A (RNAse)

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: phosphate buffered saline (PBS), pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Jeffries CM, Svergun DI
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 16 nm3

...M3 – ...midomethylated ribonuclease A (unfolded RNAse) - with and without urea

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: ...mM HCl, pH: 1
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Jeffries CM, Svergun DI
RgGuinier 2.3 nm
Dmax 9.0 nm

SASDDN3 – ...m albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: ...m albumin , 66 kDa Bos taurus protein
Buffer: ...mM HEPES, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
...Jeffries CM, Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDJF4 – Synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23 experimental SAS data
CORAL model
Sample: Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Buffer: ...mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...May 5
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 ...mmunications 11(1) (2020)
...M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 22 nm3

SASDJG4 – ...main (RBD)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data
SASREF model
Sample: ...main) monomer, 29 kDa Severe acute respiratory … protein
Buffer: ...mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...May 1
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 ...mmunications 11(1) (2020)
...M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 3.0 nm
Dmax 13.1 nm
VolumePorod 64 nm3

SASDJH4 – ...main (RBD) bound to the synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data
CORAL model
Sample: Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
...main) monomer, 29 kDa Severe acute respiratory … protein
Buffer: ...mM Tris 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...May 10
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 ...mmunications 11(1) (2020)
...M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C
RgGuinier 3.5 nm
Dmax 15.1 nm
VolumePorod 87 nm3

SASDB95 – ...membrane protein IcsA autotransporter

Outer membrane protein IcsA (53-758) experimental SAS data
DAMMIN model
Sample: ...membrane protein IcsA (53-758) monomer, 72 kDa Shigella flexneri protein
Buffer: ...mM Tris 150 mM NaCl 10 mM CaCl2 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...May 18
...mology/Cofilin/Acidic (VCA) Domain. ...m 292(1):134-145 (2017)
Mauricio RP, Jeffries CM, Svergun DI, Deane JE
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 103 nm3

SASDNC5 – ...mannii riboflavin biosynthesis protein RibD

Riboflavin biosynthesis protein RibD experimental SAS data
GASBOR model
Sample: Riboflavin biosynthesis protein RibD ...mer, 80 kDa ...mannii protein
Buffer: ...mM NaCl, 10 mM Tris, 1 mM DTT, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Dec 3
...mbinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
...Jeffries CM, Wilharm G
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 115 nm3

SASDND5 – ...mannii aconitate hydratase B, monomer fraction

Aconitate hydratase B experimental SAS data
GASBOR model
Sample: Aconitate hydratase B monomer, 96 kDa ...mannii protein
Buffer: ...mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Dec 3
...mbinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
...Jeffries CM, Wilharm G
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 150 nm3

SASDNE5 – ...mannii aconitate hydratase B, monomer/dimer mixed fraction

Aconitate hydratase B experimental SAS data
GASBOR model
Sample: Aconitate hydratase B , 96 kDa ...mannii protein
Buffer: ...mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Dec 3
...mbinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
...Jeffries CM, Wilharm G
RgGuinier 4.0 nm
Dmax 14.0 nm
VolumePorod 230 nm3

SASDNF5 – ...mannii transcriptional repressor NrdR (likely filamentous assembly)

Transcriptional repressor NrdR experimental SAS data
Transcriptional repressor NrdR Kratky plot
Sample: Transcriptional repressor NrdR , 19 kDa ...mannii (strain … protein
Buffer: ...mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Dec 3
...mbinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
...Jeffries CM, Wilharm G
RgGuinier 19.9 nm
Dmax 92.6 nm

SASDHP5 – ...m (hexameric transcriptional activator of type VI secretion gene expression)

Regulator Of Virulence interconnected with the Csr system experimental SAS data
CORAL model
Sample: ...m ...mer, 175 kDa Yersinia pseudotuberculosis protein
Buffer: ...mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2016 Jun 18
...meric transcriptional activator promoting type VI secretion gene expression PLOS Pathogens 16(9):e1008552 (2020)
...M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J
RgGuinier 4.2 nm
Dmax 12.4 nm
VolumePorod 280 nm3

SASDWQ5 – ...m Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data
CORAL model
Sample: ...me polyprotein monomer, 17 kDa ...murine encephalomyelitis … protein
Buffer: ...mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2021 Apr 26
...mal frameshifting (2025)
...Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWR5 – ...mulatory element RNA

Stimulatory element RNA experimental SAS data
SREFLEX model
Sample: ...mulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: ...mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2021 Apr 26
...mal frameshifting (2025)
...Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 19 nm3

SASDWS5 – ...m Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: ...me polyprotein monomer, 17 kDa ...murine encephalomyelitis … protein
...mulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: ...mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2021 Apr 26
...mal frameshifting (2025)
...Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3

SASDHY5 – ...mbinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data
Photinus pyralis firefly luciferase Kratky plot
Sample: Photinus pyralis firefly luciferase , 61 kDa Photinus pyralis protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDJY5 – ...monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: ...monophosphate dehydrogenase , 59 kDa ...ma brucei brucei protein
Buffer: ...mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHZ5 – ...monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: ...monophosphate dehydrogenase , 59 kDa ...ma brucei brucei protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDJZ5 – ...monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data
Inosine-5'-monophosphate dehydrogenase Kratky plot
Sample: ...monophosphate dehydrogenase , 59 kDa ...ma brucei brucei protein
Buffer: ...mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH26 – ...mbinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: ...cysteine protease , 37 kDa ...ma brucei protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH36 – ...major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: ...major protein , 19 kDa Neurospora crassa protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH46 – ...mock recombinant baculovirus (rBV)

mock recombinant baculovirus experimental SAS data
mock recombinant baculovirus Kratky plot
Sample: mock recombinant baculovirus , 0 kDa unidentified baculovirus
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells experimental SAS data
High Five insect cells Kratky plot
Sample: High Five insect cells , 0 kDa Trichoplusia ni
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH66 – ...major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: ...major protein , 19 kDa Neurospora crassa protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH76 – ...mbinantly expressed within High Five insect cells (cell-culture serial dilution series)

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: ...cysteine protease , 37 kDa ...ma brucei protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 3
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH86 – ...mbinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: ...cysteine protease , 37 kDa ...ma brucei protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDA96 – ...me

Lysozyme C experimental SAS data
DAMMIF model
Sample: ...me C monomer, 14 kDa Gallus gallus protein
Buffer: ...mM Sodium Acetate/HEPES, pH: 6.8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Feb 17
...Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. ...Methods 12(5):419-22 (2015)
...Jeffries CM, Svergun DI
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 24 nm3

SASDH96 – ...mbinantly expressed within Sf9 insect cells

Cathepsin B-like cysteine protease experimental SAS data
Cathepsin B-like cysteine protease Kratky plot
Sample: ...cysteine protease , 37 kDa ...ma brucei protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDAA6 – ...man serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: ...man serum albumin monomer monomer, 66 kDa ...mo sapiens protein
Buffer: ...mM HEPES, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2014 Jan 22
...Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. ...Methods 12(5):419-22 (2015)
...Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDHA6 – ...major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: ...major protein , 19 kDa Neurospora crassa protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDAB6 – ...merase

Xylose Isomerase experimental SAS data
DAMMIF model
Sample: ...merase ...mer, 173 kDa ...myces rubiginosus protein
Buffer: ...mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2013 Dec 10
...Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. ...Methods 12(5):419-22 (2015)
...Jeffries CM, Svergun DI
RgGuinier 3.2 nm
Dmax 9.1 nm
VolumePorod 234 nm3

SASDHB6 – ...major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: ...major protein , 19 kDa Neurospora crassa protein
Buffer: ...mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Nov 21
...mall-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDAF6 – ...main of Kgp gingipain

K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data
BUNCH model
Sample: ...modules of lysine-specific (Kgp) gingipain monomer, 74 kDa ...monas gingivalis W83 protein
Buffer: ...mM TRIS 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15
...modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Mol Microbiol 81(5):1358-73 (2011)
...Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA
RgGuinier 4.3 nm
Dmax 14.7 nm

SASDAG6 – ...mains of Kgp gingipain

K1K2 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data
BUNCH model
Sample: ...modules of lysine-specific (Kgp) gingipain monomer, 38 kDa ...monas gingivalis W83 protein
Buffer: ...mM TRIS 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15
...modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Mol Microbiol 81(5):1358-73 (2011)
...Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA
RgGuinier 2.9 nm
Dmax 9.5 nm

SASDVV6 – Extended single alpha-helix (SAH) region of Drebrin

Drebrin experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Drebrin monomer, 10 kDa ...mo sapiens protein
Buffer: ...mM NaH2PO4, 3 mM Na2HPO4, 50 mM NaCl, pH: 6
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2023 Jul 7
...mic Interchange of Local Residue-Residue Interactions in the Largely Extended Single Alpha-Helix in Drebrin ...mical Journal (2025)
...Jeffries C, Permi P, Gáspári Z
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 18 nm3

SASDPF7 – ...MBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: ...mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 16
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDPG7 – ...MBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: ...mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Jul 8
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDPH7 – ...MBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: ...m Aspergillus flavus ...mer, 137 kDa Aspergillus flavus protein
Buffer: ...mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 15
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 3.2 nm
Dmax 8.8 nm
VolumePorod 221 nm3

SASDPJ7 – ...MBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: ...m Aspergillus flavus ...mer, 137 kDa Aspergillus flavus protein
Buffer: ...mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Jul 8
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 214 nm3

SASDPK7 – ...merase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: ...merase ...mer, 173 kDa ...myces rubiginosus protein
Buffer: ...mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 15
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 3.2 nm
Dmax 14.1 nm
VolumePorod 228 nm3

SASDPL7 – ...merase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: ...merase ...mer, 173 kDa ...myces rubiginosus protein
Buffer: ...mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Jul 8
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 3.3 nm
Dmax 14.1 nm
VolumePorod 233 nm3

...M7 – ...MBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa ...ma longibrachiatum protein
Buffer: ...mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 16
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 1.6 nm
Dmax 4.8 nm
VolumePorod 25 nm3

SASDPN7 – ...MBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa ...ma longibrachiatum protein
Buffer: ...mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Jul 8
...mall-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
...ment Blanchet, Melissa Graewert, Cy M Jeffries, ...mitri Svergun
RgGuinier 1.6 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDJS7 – ...m-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: ...mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Aug 22
...mation can be regulated by mutating the PLD ...mistry :102631 (2022)
...m L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDKS7 – ...MgCl2

HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase ...mer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: ...mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 15
...Movement of the Metal Cofactor between Active and Resting State ...mie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.0 nm
VolumePorod 224 nm3

SASDJT7 – ...m-associated RNA chaperone-like protein (PARCL) at pH 6.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: ...mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2020 Aug 22
...mation can be regulated by mutating the PLD ...mistry :102631 (2022)
...m L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3

SASDKT7 – ...mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116A) experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase (S116A) ...mer, 165 kDa ...monas wittichii (strain … protein
Buffer: ...mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 15
...Movement of the Metal Cofactor between Active and Resting State ...mie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.1 nm
VolumePorod 222 nm3

SASDKU7 – ...mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116C) experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase (S116C) ...mer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: ...mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Dec 15
...Movement of the Metal Cofactor between Active and Resting State ...mie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.0 nm
VolumePorod 223 nm3

SASDHF8 – ...mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K Rg histogram
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa ...mo sapiens protein
Buffer: ...mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...Mar 13
...mment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method ...mical Theory and Computation (2021)
...Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.4 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDHG8 – ...mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa ...mo sapiens protein
Buffer: ...mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...Mar 13
...mment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method ...mical Theory and Computation (2021)
...Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.4 nm
VolumePorod 3 nm3

SASDHH8 – ...mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa ...mo sapiens protein
Buffer: ...mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...Mar 13
...mment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method ...mical Theory and Computation (2021)
...Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDFT8 – ...mer) bound to the DNA response element HoxB13 DR0

Retinoic acid receptor alpha, RARRetinoic acid receptor RXR-alphaDNA response element HoxB13 DR0 experimental SAS data
CORAL model
Sample: Retinoic acid receptor alpha, RAR monomer, 41 kDa Mus musculus protein
Retinoic acid receptor RXR-alpha monomer, 38 kDa Mus musculus protein
...ment HoxB13 DR0 monomer, 10 kDa DNA
Buffer: ...mM Tris, pH 8, 150 mM NaCl, 5% v/v glycerol, 1 mM CHAPS, 4 mM MgSO4, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2014 Jan 19
Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR–RXR Nucleic Acids Research (2020)
...McEwen A, Bourguet M, Przybilla F, Peluso-Iltis C, Poussin-Courmontagne P, Mély Y, Cianférani S, Jeffries C, Svergun D, Rochel N
RgGuinier 3.8 nm
Dmax 14.5 nm
VolumePorod 132 nm3

SASDFU8 – ...mer) bound to the DNA response element F11r DR5.

Retinoic acid receptor alpha, RARRetinoic acid receptor RXR-alphaDNA response element F11r DR5 experimental SAS data
CORAL model
Sample: Retinoic acid receptor alpha, RAR monomer, 41 kDa Mus musculus protein
Retinoic acid receptor RXR-alpha monomer, 38 kDa Mus musculus protein
...ment F11r DR5 monomer, 13 kDa DNA
Buffer: ...mM Tris, pH 8, 150 mM NaCl, 5% v/v glycerol, 1 mM CHAPS, 4 mM MgSO4, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2014 Jan 19
Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR–RXR Nucleic Acids Research (2020)
...McEwen A, Bourguet M, Przybilla F, Peluso-Iltis C, Poussin-Courmontagne P, Mély Y, Cianférani S, Jeffries C, Svergun D, Rochel N
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 130 nm3

SASDHG9 – ...m Thermoproteus uzoniensis - apo form

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa ...moproteus uzoniensis protein
Buffer: ...mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 27
...meric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 77 nm3

SASDHH9 – ...m Thermoproteus uzoniensis - with magnesium

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa ...moproteus uzoniensis protein
Buffer: ...mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 27
...meric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 76 nm3

SASDHJ9 – ...m Thermoproteus uzoniensis - with magnesium and trehalose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa ...moproteus uzoniensis protein
Buffer: ...mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 27
...meric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 76 nm3

SASDHK9 – ...m Thermoproteus uzoniensis - with magnesium and UDP-glucose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa ...moproteus uzoniensis protein
Buffer: ...mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Sep 27
...meric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 73 nm3

SASDEX9 – ...mer in HEPES isolated using SEC-SAXS

HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase ...mer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: ...mM HEPES,, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2018 Nov 23
...MIX , a tool to decompose inline chromatography SAXS data from partially overlapping components Protein Science (2021)
...M, Jeffries C, Fukuda M, Cheremnykh T, Volkov V, Svergun D
RgGuinier 3.3 nm
Dmax 9.4 nm
VolumePorod 233 nm3

SASDFP8 – ...m bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data
DAMMIN model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: ...mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 5
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 37 nm3

SASDEY9 – ...main fragment, N5N4

Collagen, type VI, alpha 3 experimental SAS data
Collagen VI von Willebrand factor (VWA) double-domain fragment, N5N4 Rg histogram
Sample: Collagen, type VI, alpha 3 monomer, 44 kDa Mus musculus protein
Buffer: ...mM TRIS, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2018 Apr 20
...main array: adaptability and functional implications of myopathy causing mutations ...mistry :jbc.RA120.014865 (2020)
...mon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 66 nm3

SASDEZ9 – ...main fragment, N6N3

Collagen, type VI, alpha 3 experimental SAS data
Collagen VI von Willebrand factor (VWA) four-domain fragment, N6N3 Rg histogram
Sample: Collagen, type VI, alpha 3 monomer, 93 kDa Mus musculus protein
Buffer: ...mM TRIS, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2018 Nov 4
...main array: adaptability and functional implications of myopathy causing mutations ...mistry :jbc.RA120.014865 (2020)
...mon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R
RgGuinier 4.1 nm
Dmax 15.6 nm
VolumePorod 143 nm3

SASDFQ8 – ...m albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...m albumin monomer, 66 kDa Bos taurus protein
Buffer: ...mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 5
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDJJ4 – ...main

Collagen alpha-3(VI) chain, N2 domain experimental SAS data
DAMMIN model
Sample: ...main monomer, 22 kDa ...mo sapiens protein
Buffer: ...mM TRIS, pH 7.4, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at ...MBL P12, PETRA III on ...May 4
...main array: adaptability and functional implications of myopathy causing mutations ...mistry :jbc.RA120.014865 (2020)
...mon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 40 nm3

SASDFR8 – ...m albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...m albumin ...mer, 133 kDa Bos taurus protein
Buffer: ...mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 5
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDFS8 – ...multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 ...mer, 147 kDa ...myces cerevisiae protein
Buffer: ...mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 5
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDFN8 – ...m horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain ...mer, 479 kDa Equus caballus protein
Buffer: ...mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2019 Apr 5
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3

SASDF99 – ...m albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...m albumin monomer, 66 kDa Bos taurus protein
Buffer: ...mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at ...MBL P12, PETRA III on 2017 Apr 23
...matography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3