Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Espinosa-Vinals

SASDL62 – The truncated RTX domain of adenylate cyclase toxin CyaA - construct RTX-1 (amino acids 1132-1294 and 1562-1681 of CyaA)

hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) monomer, 30 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

Almost half of the RTX domain is dispensable for complement receptor 3 binding and cell-invasive activity of the adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	41	nm³

SASDL72 – The truncated RTX domain of adenylate cyclase toxin CyaA - construct RTX-2 (amino acids 1132-1303 and 1562-1681 of CyaA)

hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) monomer, 31 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	37	nm³

SASDPP3 – RTX719. Double-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)

Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

RTX719. Double-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 15

Acyl chains stabilize the acylated domain and determine the receptor-mediated interaction of the Bordetella adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L

R_g^Guinier	3.4	nm
D_max	14.5	nm
Volume^Porod	67	nm³

SASDPQ3 – RTX719-K860R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)

Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

RTX719-K860R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 16

R_g^Guinier	5.0	nm
D_max	25.8	nm
Volume^Porod	178	nm³

SASDPR3 – RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)

RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 17

R_g^Guinier	5.3	nm
D_max	26.3	nm
Volume^Porod	187	nm³

SASDPS3 – proRTX719. Non-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)

Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data

proRTX719. Non-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram

Sample:	Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer:	50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 22