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9 hits found for Falke

SASDPF2 – Structure of an extracellular superoxide dismutase from Onchocerca volvulus

Extracellular superoxide dismutase [Cu-Zn] experimental SAS data
GASBOR model
Sample: Extracellular superoxide dismutase [Cu-Zn] dimer, 34 kDa Onchocerca volvulus protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development Acta Crystallographica Section F Structural Biology Communications 78(6) (2022)
...Falke S
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 55 nm3

SASDRN5 – Nucleolar RNA Chaperone-Like Protein 1 (NURC1)

AT5g04600/T32M21_200 experimental SAS data
GASBOR model
Sample: AT5g04600/T32M21_200 monomer, 25 kDa Arabidopsis thaliana protein
Buffer: 50 mM HNa2PO4, 300 mM NaCl, 5% glycerol (v/v), 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 22
Structural and functional analysis of a plant nucleolar RNA chaperone-like protein. Sci Rep 13(1):9656 (2023)
...Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 66 nm3

SASDJS7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
...Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
...Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3

SASDAY8 – Human alphacoronavirus non-structural protein Nsp10 in the presence of Zn

HCoV-229E Non-structural protein 10 experimental SAS data
CORAL model
Sample: HCoV-229E Non-structural protein 10 monomer, 15 kDa Human coronavirus 229E protein
Buffer: 25 mM HEPES 280 mM NaCl 2 mM DTT 500 µM ZnCl2, pH: 7.6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 25
Human alphacoronavirus non-structural protein Nsp10
Sven Falke, Al Kikhney
RgGuinier 1.7 nm
Dmax 5.8 nm

SASDA39 – HCoV-229E Nsp10 in the absence of Zn

HCoV-229E Non-structural protein 10 experimental SAS data
CORAL model
Sample: HCoV-229E Non-structural protein 10 monomer, 15 kDa Human coronavirus 229E protein
Buffer: 25 mM HEPES 400 mM NaCl 1 mM EDTA 5% glycerol 40 mM NaH2PO4, pH: 7.9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2011 Sep 8
Human alphacoronavirus non-structural protein Nsp10
Sven Falke, Al Kikhney
RgGuinier 1.9 nm
Dmax 6.9 nm

SASDA49 – Cation-free slp-B53

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 5.8 nm
Dmax 22.0 nm
VolumePorod 495 nm3

SASDA59 – slp-B53 with Ca2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Ca2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.4 nm
Dmax 28.1 nm
VolumePorod 609 nm3

SASDA69 – slp-B53 with Mg2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Mg2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
...Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.6 nm
Dmax 29.0 nm
VolumePorod 597 nm3