Small Angle Scattering Biological Data Bank SASBDB

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12 hits found for Karen Manalastas-Cantos

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1 experimental SAS data

MONSA model

Sample:	Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20

Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos

R_g^Guinier	5.2	nm
D_max	17.7	nm
Volume^Porod	296	nm³

SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)

Condensin complex subunit 1Condensin complex subunit 2, 336-418 experimental SAS data

MONSA model

Sample:	Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein Condensin complex subunit 2, 336-418 monomer, 9 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20

Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos

R_g^Guinier	5.0	nm
D_max	16.9	nm
Volume^Porod	279	nm³

SASDJ32 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 225-418 (Brn1L)

Condensin complex subunit 1Condensin complex subunit 2, 225-418 experimental SAS data

MONSA model

Sample:	Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20

Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos

R_g^Guinier	4.9	nm
D_max	15.9	nm
Volume^Porod	309	nm³

SASDJ42 – Pentameric assembly of condensin complex subunits 1 and 2 (Ycs4-Brn1L-Brn1C) with subunits of the SMC hinge domain-containing protein (Smc4hd)

Condensin complex subunit 1Condensin complex subunit 2, 225-418Condensin complex subunit 2, 776-898SMC hinge domain-containing protein, 263-466SMC hinge domain-containing protein, 1367-1542 experimental SAS data

MONSA model

Sample:	Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein Condensin complex subunit 2, 776-898 monomer, 14 kDa Chaetomium thermophilum protein SMC hinge domain-containing protein, 263-466 monomer, 22 kDa Chaetomium thermophilum protein SMC hinge domain-containing protein, 1367-1542 monomer, 20 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20

Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos

R_g^Guinier	5.1	nm
D_max	17.9	nm
Volume^Porod	355	nm³

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein experimental SAS data

DAMMIF model

Sample:	Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	4.6	nm
D_max	15.6	nm
Volume^Porod	236	nm³

SASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)

Condensin complex subunit 3-like proteinCondensin complex subunit 2 experimental SAS data

DAMMIF model

Sample:	Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein Condensin complex subunit 2 monomer, 17 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	4.3	nm
D_max	13.7	nm
Volume^Porod	230	nm³

SASDFE4 – Condensin complex subunit 3-like protein, Ycg1, at 10mg/ml (tetramer)

Condensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	8.3	nm
D_max	32.7	nm
Volume^Porod	1080	nm³

SASDFF4 – Condensin complex subunit 3-like protein, Ycg1, at 5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	6.8	nm
D_max	29.8	nm
Volume^Porod	720	nm³

SASDFG4 – Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)

Condensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	5.4	nm
D_max	19.0	nm
Volume^Porod	463	nm³

SASDFH4 – Condensin complex subunit 3-like protein, Ycg1, at 1mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	5.3	nm
D_max	18.7	nm
Volume^Porod	400	nm³

SASDFJ4 – Condensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	4.7	nm
D_max	16.1	nm
Volume^Porod	301	nm³

SASDFK4 – Condensin complex subunit 3-like protein, Ycg1, at 0.25mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data

SASREF MX model

Sample:	Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20

Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI

R_g^Guinier	4.6	nm
D_max	15.0	nm
Volume^Porod	280	nm³