The oligomeric state of chitooligosaccharide deacetylase from the marine bacterium Vibrio campbellii.

Pongnan S Robinson RC, Kamonsutthipaijit N, Fukamizo T, Suginta W, Biophys Rep (N Y) :100275 (2026) Europe PMC

SASDZZ9 – Chitooligosaccharide deacetylase (NodB) from the marine bacterium Vibrio campbellii (VhCOD) native form

Chito oligosaccharide deacetylase from Vibrio harveyi
MWexperimental 45 kDa
MWexpected 45 kDa
log I(s) 2.13×101 2.13×100 2.13×10-1 2.13×10-2
Chito oligosaccharide deacetylase from Vibrio harveyi small angle scattering data  s, nm-1
ln I(s)
Chito oligosaccharide deacetylase from Vibrio harveyi Guinier plot ln 2.13×101 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Chito oligosaccharide deacetylase from Vibrio harveyi Kratky plot 1.104 0 √3 sRg
p(r)
Chito oligosaccharide deacetylase from Vibrio harveyi pair distance distribution function Rg: 2.8 nm 0 Dmax: 8.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Chito oligosaccharide deacetylase from Vibrio harveyi PYMOL model
log I(s)
 s, nm-1
Chito oligosaccharide deacetylase from Vibrio harveyi DAMMIF model
Synchrotron SAXS data from solutions of Chitooligosaccharide deacetylase (NodB) from the marine bacterium Vibrio campbellii (VhCOD) native form in 20 mM Tris, 100 mM NaCl, pH 7.5 were collected on the BL1.3W beam line at the Synchrotron Light Research Institute (SLRI) storage ring (Nakhon Ratchasima, Thailand) using a Rayonix SX165 detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.137 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 16°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Chito oligosaccharide deacetylase from Vibrio harveyi (VhCOD)
Mol. type   Protein
Organism   Vibrio campbellii (strain ATCC BAA-1116)
Olig. state   Monomer
Mon. MW   45 kDa
 
UniProt   A7MSF4 (23-427)
Sequence   FASTA
 
PDB ID   8YFP